Coding

Part:BBa_K3698000:Design

Designed by: chi xu   Group: iGEM20_XHD-ShanDong-China   (2020-10-19)


degP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1780
    Illegal EcoRI site found at 23
    Illegal XbaI site found at 38
    Illegal PstI site found at 108
    Illegal PstI site found at 1452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 23
    Illegal SpeI site found at 1781
    Illegal PstI site found at 108
    Illegal PstI site found at 1452
    Illegal PstI site found at 1795
    Illegal NotI site found at 7
    Illegal NotI site found at 29
    Illegal NotI site found at 1788
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 23
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1781
    Illegal EcoRI site found at 23
    Illegal XbaI site found at 38
    Illegal PstI site found at 108
    Illegal PstI site found at 1452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 23
    Illegal XbaI site found at 16
    Illegal XbaI site found at 38
    Illegal SpeI site found at 1781
    Illegal PstI site found at 108
    Illegal PstI site found at 1452
    Illegal PstI site found at 1795
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

E.coli

References